European Journal of Molecular & Clinical Medicine

Introduction: Autologous fat grafting is an aesthetic and reconstructive procedure in which an individual’s own fat is harvested and injected into the soft tissues to correct contour and other abnormalities. Fat graft is considered the ideal soft tissue filler for its biocompatibility, lack of immunogenicity and availability.
Materials and Methods: There were two groups and the first 25 patients were assigned to system-AF and the subsequent 25 patients were assigned to processing via system-PF. The operating surgeons and operating room staff were adequately trained before the procedure. Tumescence solution was used with either technique based on the surgeon’s standard practice with a limit of 1 litre. Means and standard deviations were used to abstract the continuous patient characteristics. Frequencies and proportions were used to present categorical patient characteristics.
Results: All patients (n = 50) were female with a history of breast cancer. Mean age was 51.7 years (SD ± 8.3 years; range, 36-70 years). The average body mass index was 27.6 kg/m². The average follow-up was approximately 3 years (35.5 months; range, 12.4 - 47.2 months). All patients were reportedly undergone mastectomy earlier with breast reconstruction. More patients had completed 2-staged tissue expander to implant-based reconstruction (n = 32, 65%) compared to autologous reconstruction (n = 19, 38%). The majority of patients (n = 45, 90%) received chemotherapy, and less than half of patients received radiation therapy (n = 23, 45%) prior to their fat grafting. Five patients (10%) had a postoperative complication. There was 4 incidence of palpable fat necrosis per group and no fat emboli in the study population. For all patients, this was the first fat grafting procedure; 25% of patients (n = 4 in each group) went on to have additional fat grafting. All other patients demographics and reconstruction parameters were not statistically different between the 2 groups.
Conclusion: AFG is essentially served as a valuable tool for majority of the plastic surgery that is increasing in its popularity that could possibly leading to new devices that were aimed to increase the efficacy and yield of injectable fat.

Abstract: The task of the present paper is to develop scientific bases and technological
solutions for creating a combined pharmaceutical composition for normalization of intestinal
system function. The assigned task is solved by a combination of energy sorbent of
montmorillonite clay with a mixture of probiotic - Lastobacillus plantarum mal and prebiotic
inulin. The creation of such a composition is the most effective for normalization of intestinal
system function since prebiotic products create a favorable and complete nutrient environment
for maintenance and growth of the useful microbial culture of probiotics that were in
annibiosis (inactive state), and the enterosorbent of montmorillonite clay provides "adsorption
activity" for adsorption of toxic substances. The essence of the technology for obtaining a
combined pharmaceutical composition for the normalization of intestinal system function,
containing prebiotic and probiotic, as well as enterosorbent - montmorillonitic clay, in which
from dry inulin - prebiotic prepare aqueous solution, then it is sterilized by autoclaving, added
probiotic culture Lastobacillus plantarum mal, which is restored from the dried state by three
times crossing into the MRS - broth, At the same time, Lastobacillus plantarum mal is
centrifuged, cells are separated from the cultural liquid and thoroughly mixed before
lyophilisation of the substance. The suspension is mixed with a protective medium consisting
of milk, sugar and gelatine, the resulting mixture is also subjected to lyophilic drying, the dry
amorphous biomass is added to autoclaved montmorrillionite clay and the final product is
light brown with a pleasant sour-milk smell. This product serves as a sachet filling station -
sachets with additives.