Objective: This randomized controlled trial aims to investigate the effectiveness of mobile devices in alleviating separation anxiety among pediatric patients during the preoperative period. By analyzing the impact of mobile devices on emotional well-being, comfort levels, and overall experience, the study seeks to contribute valuable insights to enhancing the preoperative care of pediatric patients. Methods: Twenty pediatric patients, aged 4 to 10 years, scheduled for various surgical procedures, were randomly assigned to two groups: the Intervention Group (IG) and the Control Group (CG). The IG participants were provided with mobile devices containing age-appropriate games and content to engage with during the preoperative waiting period. The CG participants did not have access to mobile devices. Results: Anxiety levels were assessed using standardized anxiety scales, facial expression analysis, and nurse-reported observations. Comfort levels, cooperative behavior, and overall experience were also measured. Data collected from the IG and CG were compared using appropriate statistical tests to evaluate the impact of mobile device use. Conclusion: The results of this study indicated a significant reduction in anxiety levels among pediatric patients in the Intervention Group who were engaged with mobile devices during the preoperative waiting period. These patients exhibited more positive facial expressions, higher comfort levels, and increased cooperative behavior compared to the Control Group. The findings underscore the potential of mobile devices as a valuable tool in easing separation anxiety and enhancing the preoperative experience of pediatric patients. This study contributes to the growing body of research on pediatric preoperative care and highlights the role of technology in improving the emotional well-being of young patients. Further research with larger sample sizes and diverse populations is recommended to validate and extend these findings, ultimately leading to improved care practices for pediatric patients undergoing surgical treatments.