Background: Vitamin D deficiency is prevalent worldwide. Its prevalence in India ranges from 34%- 99% with most studies reporting a range of 80% to 90%. Methods: The study was conducted at Sushrut Surgical hospital, Nallasopara, Maharashtra, and 98 patients were included. The patients who presented to the OPD with symptoms of chest pain/ abdomen pain/ breast pain/ diffuse body pain/ backache were evaluated with routine clinical examination and investigations were done. According to their symptoms, protocol for chest pain like ECG, Cardiac enzymes, Chest X-ray, Stress test etc. Similarly, breast pain patients underwent clinical examination of breast and axilla and ultrasound/mammography of both the breasts was done. Abdomen pain patients were evaluated with ultrasound and gastroduodenoscopy. The patients who had no conclusive evidence of any obvious pathology were included in this study and evaluated for serum Vitamin D and Vitamin B12 levels.