Background&Method: This study is done with an aim to analyze CKMB and Homocysteine level in CRF. In this study, 200 people over the age of 20 including both sexes who had chronic renal failure were selected. These patients (subjects) were attending OPD and admitted to the ward of general medicine. In addition, 200 normal healthy individuals (control) over the age of 20, staff members, patient attendants, and volunteers were included as controls. The patients were diagnosed as chronic renal failure by the department of general medicine at index medical college hospital and research centreindore (MP) and Amaltas institute of medical sciences, Dewas (M.P.). Result:Our study was done in the clinical biochemistry laboratory with association of general medicine at index medical college hospital & research centreindore&Amaltas institute of medical sciences dewas (MP). The study included 200 chronic renal failure patients and the control group included 201 healthy individuals of both sexes between 20 to 70 years.Serum CPK-MB level in subjects and controls. It shows 41.91±16.80and 8.12±1.07 in subjects and controls, respectively. Blood Glucose level in Female subjects and controls. It shows 259.78±101.83 and 86.71±8.4 in subjects and controls, respectively. Conclusion:The mean serum concentration Homocysteine of CRF patients was high (21.51±10.10) in comparison of healthy control mean (6.03 ± 1.19). It has been found that the difference between them was also statistically significant p-value is <0.001. The mean serum concentration total cholesterol of CRF patients was high (331.73±71.80) in comparison of healthy control mean (110.17 ± 10.12). It has been found that the difference between them was also statistically significant p-value is <0.001. This study shows that there is a high risk of inflammation and cardiac diseases in CRF patients. It is very important to get tested annually to prevent CKD as well as reduce kidney failure.for cardiac and inflammatory markers, make lifestyle changes, and see your health care team regularly.