Abstract: The use of metal nanomaterials in various applications due to their tunable optoelectric properties are growing and excitement filed. These optoelectric properties of metal nanomaterials are used in various biomedical applications and are highly depend on the size of the nanostructure.[1, 2] These properties of metal nanostructure is due to the oscillation of electrons with in the materials, resulting surface plasmon resonances (SPRs) generates, that makes these materials unique from the bulk. These unique features of nanomaterial can be utilized in molecular sensing, photothermal therapy, tagging.[3-6] These optical properties can vary with the nanomaterials. Among various kind of nanomaterials gold metal nanomaterial have attracted researchers due to their potential application in the field of biomedical technologies, imaging and optical and optoelectronic devices. Moreover, the metal nanostructures are most suitable agent for imaging because of their stability under the light illumination. The surface functionalizations of these nanostructures are very facile with biological groups and chemicals that make them outstanding in the field of targeted imaging.[7] Moreover, the capabilities of nanomaterials to accommodate numerous functional groups, biomolecules, and drugs permit us to customize them for cure of various cancer cells.[8] Bio macromolecules (proteins) are the major part for living organism which carried the information encoded by the genes to the specific site. In biological organisms the self-association of proteins is a common phenomenon that sometime lead to severe side effects. So, the photophysical study on their conformational dynamics in highly crowded environment is of great importance. Therefore, it is essential to design a nanomaterials that would be accurately precise to a given cell and cellular pathway.