INTRODUCTION: The most prevalent endocrine condition in clinical practice is thyroid dysfunction, and approximately half of the population with thyroid dysfunction remains undiagnosed. In India, too the burden of thyroid diseases is significant. For normal human growth and mental development, iodine is an important micronutrient with an RDA of 100- 150μg. Iodine deficiency disorder (IDD) is a complex clinical and subclinical disorder triggered predominantly by insufficient intake of adequate iodine in foods. One of the most common micronutrient deficiencies globally is iodine deficiency. In clinical practice, thyroid gland enlargement is a common issue associated with iodine deficiency, an increase in the amount of serum thyroid stimulating hormone (TSH), natural goitrogen, smoking, and lack of selenium and iron. At the age of puberty, Goiter is characterized by the enlargement of the entire thyroid gland. It is found in patients who have either hypothyroidism or hyperthyroidism or elevated levels of TSH. The goal of the present study was to identify associations of goiter patients with thyroid hormone levels. METHODS:The cross sectional study was carried out at SMHRC, Maharashtra India during the period of 8 th January 2020 to 7th November 2020. We enrolled 144 patients (72study group and 72control group) aged 20-35 years with goiter (Thyroid dysfunction) and normal subject from Nagpur. In each patient, thyroid function test were determined. RESULTS:The outcome indicates a substantial difference between goiter and control group individuals in hypothyroid and hyperthyroid patients. The study concluded that goiter prevalence depends on the higher and lower concentration of TSH.