Abstract: Purpose: To evaluate the application of mitomycin-C on the density ofcorneal endothelialiumafter photorefractive keratectomy (PRK) in myopia patients. Methods: We use case control study to evaluate 92 eyes of 46 patients (23 cases and 23 controls) with mean age of 27.6 years for cases group and 27.4 for control group (range: 20- 40). All patients were myopic ranged from -3.00 to -6D underwent photorefractive keratectomy (PRK). The first group (cases) wastreated with single usage of mitomycin-c for 30 seconds and second group (control) group was treated without mitomycin-c.The assessment was done by specular microscope before the procedure and after 3 months postoperatively for each eye to evaluate the density in corneal endothelialium (ECD) in both each group. Results: Forty-six eyes of 23 cases and forty-six eyes of 23control patients with myopia were treated with photorefractive keratectomy. After three months of surgery the mean ECD was decreased significantly. Conclusion: The use of mitomycin-c for 30 seconds in myopic patientstreated by PRK would affectcorneal endothelial cell density after three months postoperatively.