Background: The five most common transmissible infections in all the blood banks included HIV, HBV, HCV, syphilis and malaria. This study aims to evaluate the prevalence of prevalence of transfusion transmitted infections in a tertiary care centre. Material and methods: The present study was conducted to assess the prevalence of transfusion transmitted infections in a tertiary care centre. A total number of 1200 donors were analyzed for the seroprevalence of Transfusion Transmitted Diseases over a period of 6 months. Basic information was obtained using donor form. Physical examination was done Blood was screened for HIV, HBV, HCV, syphilis and malaria by ELISA according to manufactures instructions. Results: A total number of 1200 blood donations were taken in this study. In our study, Males outnumbered females with 93.33% donations while only 6.66% donors were females. 24 (2 %) were reactive for blood transmitted infection. In our study, HBV and HCV were the major infections in blood donors and the seroprevalence were 1.5%, and 0.16% respectively. Only 0.33% was positive for malaria. Conclusion:The present study concluded that 2 % donars were reactive for blood transmitted infection. HBV and HCV were the major infections in blood donors.