Religion is a culture of faith that influences the way of life of the people in society, as we can see, each social landscape has its own identity culture that conveys the overall values and virtues of that society, In the Lower Northeast, Thailand is an area of diverse ethnic groups, so this area is culturally interesting to visit, and of course the uninterrupted flow of people. It is something that helps to promote economic expansion as well. Therefore, the objectives of this research were to study the readiness of communities and entrepreneurs on religious and cultural tourism, the guidelines to develop the potentiality of communities and entrepreneurs on religious and cultural tourism, and the impact to communities and entrepreneurs on religious and cultural tourism in the lower southeast region of Thailand. It was a qualitative research. The research method was done by 3 types comprising (1) studying from the related documents, (2) In-depth interview from 32 key informants, and (3) The data was synthesized to build the route and network, the network strengthening, and the development of communities and entrepreneurs on religious and cultural tourism in the lower southeast region of Thailand. The results were found that: 1. The readiness of the community and business operators in religious and cultural tourism consists of the following: Tourist areas, accessibility to tourist attractions, facilities (accommodation, restaurants, transportation, recreational activities, other facilities, shops), environment and participation. 2. Guidelines for developing community and business potential: Tourist attraction capacity management, facility capacity management, safety capacity management, area capacity management and tourism resource capacity management. 3. The impact of tourism was found that the environment is destroyed, such as pollution problems in buildings, waste. More and more people in the community are fighting for expensive products. Foreign culture imitation because villagers do not have awareness of cultural preservation. There were conflicts within the community lead to insecurity of the property.