Objective: The current study was conducted to determine the relationship between BNP gene polymorphisms and some hormonal variables of a group of congestive heart failure patients. Materials and Methods: The study comprised 60 people with congestive heart failure and 30 people who were healthy. The Enzyme Linked Immunosorbent Assay (ELISA) was used to detect serum hormone levels, and the Tetra ARMS-PCR technique was used to genotype the BNP gene for locus rs198389. Results: The study's findings revealed a highly significant decrease in Atrial Natriuretic Peptide (ANP) concentration, with an average of (29.879± 6.9864) and (34.074 ±5.7455) pg/ml, and a highly significant increase in the concentration of Brain Natriuretic Peptide (BNP) with an average of (123.3839 ± 5.900) and (96,583 ± 6.191) pg/ml, and a highly significant decrease in the concentration of C-type natriuretic peptide (CNP) with an average of (174.402 ± 8.943) and (261.651 ± 9.172) pg/ml respectively at the level of probability (P≤0.01) in serum Congestive heart failure as compared with those in the control group.The results of the polymorphism of the brain natriuretic peptide gene for the rs198389 locus revealed that there were differences in the frequency of alleles and genotypes in the patient group compared to the control group, but these changes were not statistically significant. The mutant GG genotype was found in a high number of patients (OR = 2.6) compared to controls, and it was dominant over the normal genotype, and with OR = 1.350 for the G mutant allele. Thus, it is considered as a risk factor for the disease. The mutant genotype was associated with highly significant decrease in the levels of Atrial Natriuretic Peptide (ANP) and a highly significant increase of both Brain Natriuretic Peptide (BNP) and C-type Natriuretic Peptide (CNP) at the level of probability (P≤0.01) in the group of patients with congestive heart failure.