Background:Circular RNAs (circRNAs) are a class of non-coding RNAs (ncRNAs) with covalently closed single-stranded structures. The epigenetic regulatory role of ncRNAs in diabetes-associated cardiometabolic diseases is still evolving. This study aimed to investigatewhether therelative expression level ofcirc. ankyrin repeat domain36 (circANKRD36) are altered in Egyptian patients with T2DM and whether they correlatedwith othercardiometabolic risk factors. Methods: case-controlled study was conducted on forty patients with T2DM, and 20 healthy subjects were included in the study. Diagnosis of T2DM was based on ADA Criteria.The expression profile of circANKRD36 was measured using quantitative realtime (qRT) PCR. Results: The relative expression levels of circANKRD36wassignificantly increased in patients with T2DM compared to control. p˂0.001*.The relative expression levels of circANKRD36 were significantly positive correlated with cardiometabolic risk factorsand linear regression test revealed that hba1c as well as BMI, were independently correlated with epigenetic markers. Our results revealed that the sensitivities and the specificities ofcircANKRD36 expression levels to diagnosis of T2DM were 87% and .99.15. Conclusion: the relative expression levels of circANKRD36were significantlyincreased in patients with T2DM.Also,the relative expression levels of circANKRD36 were significantly positive correlated to cardiometabolic risk factors.