Treating pain in the newborn is essential for many reasons: pain can be harmful due to decreased oxygenation, hemodynamic instability, and increased intracranial pressure.The International Evidence-Based Group for Neonatal Pain recommends that the combination of a variety of pharmacological and behavioral interventions during painful procedures has synergistic effects. Data were collected duringheel lance and venipuncture performed as part of routine clinical care for the estimation of glucose and bilirubin,thyroid function tests respectively. The infants in the control group (n=64) received 10% dextrose. In the experimental group, 24% sucrose solution was used. When compared to 10% dextrose, no. of babies who received sucrose and fall into NIPS score <4 category at 1 min and 3 min during the procedure were more. Hence 24% sucrose has better analgesic effect than 10% dextrose. However statistically when Fischer exact test was applied between the groups there were no significant differences between 24% sucrose and 10% dextrose