Abstract: These days, the interest of talented laborers in Malaysia has expanded dependent on the expanded computerization and prologue to the most recent innovation for the Industrial Revolution 4.0. Accordingly, the organizations need to investigate the approaches to viably connect with their representatives and use their maximum capacity by improving positive transfer of training. Literature identified motivational factor enhances the transfer of training among employees but at the same time this factor has been influenced by workplace spirituality. However, review of literature revealed that so far, no study had been conducted between workplace spirituality towards transfer of training. This present study focused on the investigation of the relationship between workplace spirituality and the transfer of training, also the mediating effect of the motivation is investigated. To achieve the objectives of this study, a quantitative research design where the questionnaire is distributed to all the respondents involved in the “standard of procedure” training at all Al-Ikhsan’s branches in Malaysia. Further, this data is statistically tested using Statistical Packages for Social Sciences for Windows Version 25 (SPSS) for empirical investigations of the relationships between workplace spirituality, motivation and transfer of training. The results of this study confirmed mediation effect of motivation on the relationship between workplace spirituality towards transfer of training. Results of the study imply that workplace spirituality is a powerful new management concept that can be revolutionize the working environment of an organization by influencing employee performance.