ABSTRACT Background: Amniotic fluid plays important rules in fetal development. variations above or below normal is associated with increase perinatal the amniotic mortality and morbidity, so we evaluate the effect of maternal position change on changing fluid position and increasing the visual of AF and might increase the accuracy of AFI. Little reports were studied before in this subject We aimed to evaluate amniotic fluid index in comparison with maternal position change along with different methods for increasing AF accuracy and subsequently neonatal outcome. Methods: A cross sectional study included 80 pregnant women (18-35) years old whose gestational age before 24 weeks of gestation, Obstetric ultrasonographic examinations were performed to measure fetal UPR, AFI, and VDP in lateral decubitus Position at 36 weeks, then follow up measurements of AFI, and SVDP were performed at 38 – 40 weeks of gestation at both supine and lateral decubitus Position. Results: There was a statistically significant increase in FUP, AFI and VDP values at 36 weeks of GA on change maternal position from supine to lateral decubitus. Also, a similar significant increase in FUP, AFI and VDP values on changing maternal position at 38-40 weeks of gestation. There was a significant negative correlation between AFI and VDP measured on lateral decubitus position at 36 weeks and incidence of AF-stained meconium and need for NICU admission also incidence of respiratory distress. Conclusion: It seems that maternal position change from supine to lateral decubitus position increases AF accuracy as increased fetal UPR, AFI and VDP subsequently enhancing pregnancy and neonatal outcome. We may infer that the VDP approach is the best method for estimating AF volume.