Background: Periodontitis is a chronic inflammatory disease that affects the supporting structures of teeth, resulting in tooth loss. The aim of the study is to evaluate and compare the effect of scaling and root planing alone and combination of scaling and root planing (SRP) with low level Gallium Aluminium Arsenide (GaAlAs) laser therapy in patients with moderate chronic periodontitis. Materials and methods: A total of 25 patients with mean age of 38.08 years (35-48 years) having chronic generalized moderate periodontitis were included in the study. A total of 4740 sites were evaluated out of which 2370 were the control sites and 2370 were the test sites. Plaque index (PI), gingival index (GI), sulcus bleeding index (SBI), periodontal probing depth (PPD), and clinical attachment level (CAL) were recorded at baseline, 1 and 3 months after the treatment. A low level diode laser with a wavelength of 810 nm, power output 0.4 W and energy density of 2 J/cm2 & 4 J/cm2 was applied to the gingival surface after periodontal treatment on the first, second, and seventh day Result: At all time points, the LLLT at test sites showed significantly more improvement in plaque index (PI), gingival index (GI), sulcus bleeding index (SBI), clinical attachment level (CAL) and periodontal probing depth (PD) levels compared to the control group (P <0.001). Conclusion: LLLT as an adjunctive therapy to non-surgical periodontal treatment is beneficial in improving periodontal status of patients with chronic periodontitis