Background: Primiparous postpartum mothers cannot independently take care of their babies due to a lack of knowledge about baby care. This study aims to determine the effect of android-based health education on the autonomy of primiparous postpartum mothers in neonatal care. Methods: The research design was a quasi-experiment with a pretestposttest design with a controlled group design with a total sample of 19 pregnant women each of TM III primigravida (Gestational Age ≥ 38 weeks) in both of the intervention group and the control group. The sampling technique used was purposive sampling. The research was conducted at Public Health Centre of Kelayan Timur and at the PBM Clinic of Mrs. NM in Kelayan Timur Banjarmasin during September - October 2019. Data were collected using pretest and post-test containing questions about digital knowledge, skills, and independence in digital observation sheets. Data analysis was carried using the Wilcoxon test and the Mann-Whitney Test. Results: The Wilcoxon Test results showed that there were differences in the knowledge of respondents in the intervention group before and after joining Android-based health education with a value of p= 0.000. The results of the Mann-Whitney Test showed that there were differences in knowledge about neonatal care between the intervention group and the controlled group with a value of p= 0.004, there were differences in skills in neonatal care in the intervention group and the controlled group with a value of p= 0.012 for the skills of bathing babies, p= 0.016 for umbilical cord care skills and p= 0.009 for correct breastfeeding skills, and there were differences in independence in neonatal care between the intervention group and the control group. These results revealed an effect of android-based health education on the autonomy of neonatal care with a value of p= 0.001 for the autonomy in bathing babies, p= 0.006 for the autonomy in caring for the umbilical cord and p= 0.017 for the autonomy in breastfeeding. Conclusion: Android-based health education affects mothers' autonomy in neonatal care during primiparous postpartum.