ABSTRACT Background: The tooth extraction process will always cause tissue damage, both hard tissue and soft tissue such as bleeding, pain, edema, and hard tissue such as loss of alveolar ridge volume after extraction. Substitutes for biological components have developed in increasing alveolar bone volume by using bone grafts. Chitosan can be used as an option. Addition of collagen membrane to post-extraction wounds can increase and protect the formation of initial blood clots to the root surface. Collagen membrane is a type of resorb able membrane that is often used. The function of this collagen membrane is increasing and maintaining blood clots and acting as scaffold for cell adhesion and growth. Purpose: To determine whether the use of collagen membranes does not slow down the chitosan adsorption speed of 150-355 µm in group O blood or not. Methods:There were chitosan samples measuring 150-355 µm were divided into two groups, namely control group, 7 samples for chitosan with gauze and 7 samples of the treatment group for chitosan with collagen membrane. Dip it in 75 ml of blood. Measurements of group O blood adsorption velocity in each group were seen for 10 minutes. Result: The result was analyzed statistically by using Mann-Whitney test with level of significance lower than 0.05 (Sig<0.05). There was significant difference between the control group and the treated group. Conclusion: There is a slowing of adsorption rate of blood type O on chitosan with membrane collagen.