Background: The emergence of novel SARS-CoV-2 variants has heightened concerns regarding disease transmission and infection control. This study aimed to assess the awareness levels of the recently identified JN1 variant among dental professionals and patients. Methods: A cross-sectional survey was conducted among 100 dental professionals and patients. A structured questionnaire assessed knowledge, perceptions, and preventive practices related to the JN1 variant. Statistical analysis was performed to evaluate awareness levels and demographic influences. Results: Dental professionals exhibited higher awareness (75%) compared to patients (40%) regarding the JN1 variant. Knowledge gaps were evident, with professionals demonstrating better understanding of transmission (85%), symptoms (60%), and preventive measures (90%) than patients (55%, 30%, and 45% respectively). Conclusion: Significant disparities exist in the awareness of the JN1 variant between dental professionals and patients. Targeted educational interventions are critical to bridge these gaps and enhance infection control measures within dental settings.