Objective: Using cell culture and tissue culture techniques, many researchers are working to elucidate the hormonal factors necessary for the structural differentiation and functional activities of ovarian tissue. Therefore, in this study, we have studied ovarian tissue culture on mesenchymal stem cells (MSCs) derived from bone marrow. Hence, in this study, we examined ovarian tissue culture on mesenchymal stem cells isolated from cultured adipose tissue. Materials and Methods: In this study, mesenchymal stem cells became isolated from adipose tissue of the mouse. After four days and monolayer formation of Mesenchymal stem cells, obtained ovarian was placed on the cultured Mesenchymal stem cells’ monolayer for 7 days. Results: The results of this study show that numbers of mature follicles for treat group compared to the control group were 61/2±1/2 and 38/1 ± 9/9, respectively which had a significant increase compared to control group (P<0/05). This shows the efficiency of tissue culture in co-culture with Mesenchymal stem cells. Also, the amount of internal solo layers of treat group (31/2 ± 5/2) had no significant difference compared to the control group (24/1 ± 7/9). Conclusion: According to the results yielded regarding the number and quality of obtained follicles, it seems that this method is efficient and of high importance in producing mature follicles, and subsequently highquality oocytes and embryo.