Abstract Hepatitis C virus is one of the public health disorders in the world. It’s bloodborne, causes acute disease and may extend to chronic infection, if HCV is untreated, it can progress and lead to fibrosis, cirrhosis and cancer of the liver and then death. Since the virus targets the immune system, here in this finding two cytokines (IL-6 and TGFᵦ-1) and two apoptosis-related proteins (PD-1 and STAT3) were tested serologically by Enzyme LinkedImmunosorbant Assy (ELISA) technique to detect the changes in their levels during the infection. The results revealed that IL-6 and TGFᵦ-1were abnormally raised in patients with HCV compared with healthy peoples (P<0.001). Apoptosis-related proteins involved in this study were PD-1 and STAT3, both were also elevated in HCV patients rather than control (P<0.001). The age group (46-55) of patients was the most group displayed higher levels in IL6, TGFᵦ-1, PD-1 and STAT3. In conclusion, IL-6 was good indicator on the liver inflammation during HCV, TGFᵦ-1stimulates the transformation of the infected hepatocytes to be fibrotic, cirrhotic or cancer cells. PD-1 considered as a checkpoint for the impairment of T cell during infection, continuous stimulation of STAT3 by IL-6 and other factors contribute to HCV progression.