Abstract: This study was carried out commercial animal field in Aboalkassib village/ Basrah. to investigate the influence of injected of oxytocin hormone on milk production and some biochemical parameters (glucose, cholesterol, total protein and albumin) and some hormones concentrations ( prolactin, TSH and progesterone) in the Iraqi sheep and goats . A total of 40 dry adult Arabi ewes and local black female goats (20 for each type of animals) aged between 2.5-3 years old were divided randomly into two groups: 10 ewes as a control group and 10 ewes treated with oxytocin hormone at dose 12 ml/head. Also, 10 female goats as a control group and 10 female goats treated with oxytocin hormone at the same dose by injecting in intramuscular at 6:00 o`clock in the morning. The results showed significant (P<0.05) increase in milk yield in local black goats, but not in sheep, during the morning and evening periods milking when treated animals with oxytocin hormone at dose 12 ml/head in intramuscular, Also, the results showed that the concentrations of glucose (after 30min. of injection), cholesterol and prolactin (after 1h. of injection) were significant (P<0.05) increase in the blood of sheep and goats. Glucose concentration was higher in sheep compared with goats except after 30min. of the injection, also, the level of cholesterol was higher in sheep compared with goats in all periods. The concentration of progesterone decreased significantly (P<0.05) in both types of animals after oxytocin injection during all periods. Sheep and goats treated with oxytocin were not significantly different in the concentrations of total protein, albumin and TSH hormone.