Background: Aims: The objective of study is to Study the Microbiological flora of lower female genital tract in patients complaining of Vaginal discharge. Materials and methods: A total of 100 women of reproductive age group with the complaints of vaginal discharge were selected for this study at random after applying the criteria mentioned above. With the prior consent, a comprehensive history, general examination, and gynecological examination were initially carried out. After making a clinical diagnosis, a sterilized sims speculum was inserted into the vagina to visualize the vagina and cervix. Results: Bacterial Vaginosis was found in high frequency in the age group of 31-40 years (58.6%) .The most common age group affected by vaginal candidiasis was 20-30( 44.8%) .Trichomoniasis was detected in the highest rate at the age group of 31-40years (60%).Normal flora was found seen in high frequency in the age group of >50 (33.3%) followed by 31-40(26.6%). Mixed infection of Candida and BV was seen in mainly in the age group 20-30 and 31-40 (40%) followed by 41-50 (20%). 85 (85%) cases were organism positive and 15(15%) cases showed normal Flora. Among 100 patients, BV (46%) was the most common microbiological cause of abnormal vaginal discharge, followed by vaginal candidiasis (29%), trichomoniasis (5%), combined infection (Candida and BV) (5%). Characteristics of vaginal discharge of patients differed according to pathogenic agents. The pH of vaginal secretions was >5 in a large proportion of examined cases which included women with BV and TV, but women with Candida infection, the pH was slightly below the normal value. Whiff amine test was positive in most of the cases, most of them having BV. Vaginal swab for culture and sensitivity showed prevalence of micro-organism associated mainly with BV.