Background: Laparoscopy involves the visualization of abdominal and pelvic organs after creating an artificial pneumoperitoneum. Present study was aimed at to study effects of various insufflating agents during general anaesthesia and correlation of acid base and blood gas changes during laparoscopy to determine ideal insufflating agent amongst CO2, O2 and N2O at a tertiary hospital. Material and Methods: Present study was single-center, comparative study, conducted in female patients from age group of 20-40 years in ASA Class – I/ II undergoing diagnostic laparoscopy and laparoscopic tubal ligation. 105 patients were divided in three groups of 35 patients each as Group- I (oxygen as the insufflating agent), Group II (Nitrous oxide as the insufflating agent) & Group III (carbon dioxide as the insufflating agent).Results: Mean age, body weight & types of procedures were comparable among all groups & no significant statistical difference was noted