ABSTRACT Pyogenic infections are the challenges in the hospital patients invite to other nosocomial infections.It is also life threatening and required appropriate antibioticstotreatthesepyogenicbacterialinfections. Multi drug resistance is accountable to make chemotherapy more difficult to manage the bacterial pyogenic infections. Objective: To determine the antimicrobial susceptibility pattern of bacterial isolates from pyogenic infection. Methods:A study was conducted on samples collected differentwardsofthehospital.Pussamplewascollectedthroughaspirationandsterileswabs.The clinical specimens were processed immediately after collection to isolate and identification of the pyogenic bacteria Further AST was conducted by automated technique as well as Kirby-Bauer method by following the recommendedprocedures. Result: Overall 103 specimens were collectedand 44 samples were isolated out of them, showinganisolationrateof42.7%. Outof44positivesamples19weregrampositive(43.2)and25were gramnegative(56.2%).ThemajorbacteriaisolatedfrompuswasStaphylococcusaureus14(32%) , followedbyKlebsiellapneumonia6(13%)andEnterobacterspp6(13%),Escherichiacoli5(11%), Pseudomonas aeruginosa 4 (9%), Acinetobacter baumannii 2 (6%), Coagulase negative staphylococci 3 (8%), Enterococcus spp. 2 (4%). The remaining isolates were nonbacterial microbial infections. Conclusion: Emerging of MRbacterial strainsis the main focusfor thetreatment of pyogenic infections.Major challenges of pyogenic infections are due to Gramnegative bacteria which are followed by Gram positive bacteria such as Staphylococcus aureus. The change in antimicrobial pattern of antibioticsis tested by antibiotic sensitivity testing to treat the challenge in treating these conditions.