Iron deficiency anaemia increased when iron intake concentrations, iron reserves, and iron loss by the body was inadequate to completely sustain erythrocyte development. Anemia with iron deficiency rarely causes death, but the effect on human health is severe. This disorder is quickly diagnosed and treated in developed countries, but it is often ignored by doctors. On the other hand, in underdeveloped nations, it is a health issue that affects large portions of the population.Hemoglobin A1C (HbA1c) represents the glycemic status of the patient over the previous three months. Previous studies have reported that, independent of glycemia, iron deficiency can increase HbA1C concentrations. Aim:This study is aimed to analyze the effect of iron deficiency anemia on HbA1c levels inanaemicpopulation and compare with healthy individuals. MATERIAL AND METHODS: This study included 50 patients of iron deficiency anemia and 50 healthysubjectsas the control group. This is a cross-sectional investigation wherein cases were out-patients. Patients went to the Directly Observed Treatment Short-course focus in the Dept. of General Medicine, DattaMeghe Medical college, SMHRC, Nagpur in collaboration with JNMC, ABVRH (DattaMegheInstitute of MedicalSciences Deemed To Be University), Sawangi, Wardha, Maharashtra. RESULT:HBA1cour results suggested that IDA was associated with higher concentrations of HbA1c.The mean HbA1c of cases was 6.81 % while it was 5.04 % in healthy control. Application of statistical test shows that the difference of HbA1c between case and control group was statistically significant.CONCLUSION:In this study, we observed a positive association between IDA and elevated HbA1C level in non diabetic population. HbA1C rises with severity of anaemia. In iron depletion, this spurious elevation of HbA1C contributes to over or under diagnosis of diabetes at the cutoff point (6.5 percent).