Background: To assess the overall accuracy of intra-operative diagnosis of ovarian tumors by imprint cytology technique and to compare those cases with histopathological diagnosis and to determine whether the addition of cytologic preparations provides useful information in the intra-operative management.Aims and Objectives: To establish the validity and reliability of imprint cytology and its accuracy in intra-operative diagnosis of ovarian tumors and to get an overview of the incidence of ovarian malignancies in our hospital. Materials and Methods: A Prospective study of 2 years conducted from November 2011 to October 2013. The study includes all cases of ovarian tumors and excludes patients who have received treatment and cases of functional ovarian cysts. Intra-operative imprint cytology diagnosis was compared with histopathological report.Results: A total of 30 cases were studied. Age range of the patients was 13 to 69 years. Out of 30 cases, 2 cases were excluded. On histopathological diagnosis out of 28 cases, 18 were benign, 1 was borderline,8 were malignant and 1 case with no tumor( Infected cyst) . On comparing the imprint cytology diagnosis with histopathological diagnosis, 25 out of 28 cases were concordant and the diagnostic accuracy of imprint cytology was 96.4%.Conclusion: Imprint cytology is a less expensive, simple and a quick method of diagnosis. Unlike frozen section it does not affect the utility of the specimen for histopathology. It is helpful especially in young patients who need conservative surgery in order to preserve fertility.