Introduction : Post-operative wound infection is an infection that develops within 30 days after an operation (OR) within one year if an implant was placed and infection appears to be related to the surgery. These infections number approximately 500,000 per year, among an estimated 27 million surgical procedures and account for approximately one quarter of the estimated 2 million nosocomial infections each year. Post operative wound infection remains the major source of illness and one of the cause of death in surgical patient. Aims and objectives : To isolate and identify the bacterial pathogens from the infected surgical site, to determine the Antibiotic susceptibility pattern of the isolated pathogens and to study the clinical profile and to identify the risk factors in the causation of surgical site infection. Material and methods : This is a retrospective study ,conducted in the department of Microbiology, Osmania general hospital, Hyderabad , between June 2015 to May 2016.