Non-fermenting Gram-negative bacilli (NFGNB) are emerging as important causes of blood stream infections (BSI) and they are a major cause of morbidity and mortality worldwide. Presence of intrinsic resistance of NFGNB to antimicrobial compounds makes the treatment of BSIs caused by them difficult and expensive. The aim of this study was to assess frequency and antibiotic susceptibility pattern of non-fermenting gram-negative rods isolated from blood culture of patients. A total of 3900 blood samples were received in the Department of Microbiology during 6 months period. All samples were processed according to standard microbiological procedures. 131 NFGNB were identified out of which the most common non-fermenters isolated were Acinetobacter sp. (90) followed by Pseudomonas aeruginosa (41). Most of the nonfermenters were multi drug resistant. Imipenem showed good sensitivity. This study underlines the need to identify NFGNB in tertiary care hospitals and to monitor their susceptibility pattern to guide the clinician for better care and management of patients. Improved antibiotic stewardship and strict infection control measures especially hand washing need to be implemented to prevent emergence and spread of multidrug resistant NFGNB in health care settings.