The studies have shown that bacterial pathogens differ in chronic and acute dacryocystitis. Acute dacryocystitis is caused by gram negative rods. In chronic dacryocystitis mixed flora is isolated. The percentage of culture positive was found to be higher in chronic dacryocystitis with single or mixed growth. All patients included in the study underwent basic evaluation as mentioned in the standard proforma after obtaining written informed consent. Routine ophthalmic examination was conducted by the investigator, including slit lamp examination, paying special attention to the presence of discharge and epiphora. In the present study bacterial growth was seen in 42 (84%) cases. Gram positive organisms were isolated in 27 (54%) cases and Gram negative organism in 13 (26%) cases. 2 (4%) cases showed mixed growth pattern. Staph Aureus and CONS accounted for 22% each and Streptococcus 10% cases. Among Gram negative organisms Klebsiella was isolated in 10% cases. Citrobacter and Pseudomonas were isolated in 6%.