Subfertility (also commonly known as infertility), which affects one in six couples, is the failure to manifest a clinical pregnancy after 12 months of regular, unprotected intercourse. Even though genetic, environmental and lifestyle factors play a pivotal role in subfertility, the etiology of the disease still remains enigmatic. The extent of somatic DNA damage and oxidative stress among subfertile subjects was studied. TORCH infection screening and several clinical parameters were also analyzed among 74 subfertile females and 45 age and sex-matched healthy individuals as control. The study concluded that subjects with subfertility showed increased oxidative stress and associated somatic DNA damages along with varying degrees of infections.