Introduction: Autologous Platelet Rich Plasma injection is now widely accepted treatment in the treatment of lateral epicondylitis. Prior studies on PRP injection treatment in lateral epicondylitis have not emphasized on the injection technique used to deliver PRP to the site of maximum tenderness on lateral epicondyle. PRP can be delivered by either single injection at the point of maximum tenderness or by peppering technique at the point of maximum tenderness. Which injection technique is more effective in treating lateral epicondylitis needs to be further studied. Aims and Objective: To compare the outcome of treatment of lateral epicondylitis with PRP with single injection technique and peppering injection technique in terms of improvement in functional outcome and pain. Materials and Method: A comparative study was conducted in 62 patients with lateral epicondylitis treated with PRP injection by single injection technique (31 patients) and PRP injection by peppering injection technique (31 patients).Patients were followed up at the 2nd week, 4th week 8th week and 12th week and assessed with Patient Rated Tennis Elbow Evaluation (PRTEE) Score and Visual Analog Pain Scale (VAS) Score during follow up. Repeated measures of ANOVA was used determine the statistical significance of scores during follow up in both groups. The mean scores of PRTEE and VAS score with single injection technique were compared with that of prepping injection technique using unpaired t test.