Background: Liver cirrhosis (LC) is an irreversible condition which results from necrosis of hepatocytes with loss of reticular network and nodular regeneration of residual liver tissue. This study was undertaken to assess the relation between serum prolactin levels and the severity of the liver cirrhosis.Materials & methods: This cross-sectional observational study was conducted during one calendar year at a tertiary care center in Western India among 50 cases of established liver cirrhosis. Serum Prolactin was estimated using ADVIA Centaur® CP Immunoassay System (Siemens®) by Chemiluminescent technique. The modified Child Pugh score was calculated for each study participant. The patients were categorized into Classes A, B or C based on the score obtained. Hepatic encephalopathy was diagnosed and graded as per West Haven classification system. The grade I-II were taken as mild and grade III-IV as advanced hepatic encephalopathy for calculation of the modified Child-Pugh score. Ascites was graded as mild, moderate and severe. All the data were stored in excel sheet using Microsoft® Office 2007. Results: The mean serum prolactin, serum albumin, serum Bilirubin and INR were 48.1±26.8 ng/ml, 2.8±0.6 g/dl, 4.8±4.9 mg/dl and 2.2±1.0 respectively. (Table No. 1) The mean serum prolactin level in alcoholic cirrhosis (49.2±25.1 ng/ml) does not statistically differ from non-alcoholic cirrhosis (46.6±29.4 ng/ml) cases (p > 0.05). The mean serum prolactin level was statistically significantly higher among cases in Modified Child Pugh Class-C (68.91 ± 17.80 ng/ml) compared to Class B (33.26 ± 7.41 ng/ml) and Class A (10.03 ± 4.01ng/ml) cases. The mean serum prolactin level was 81.36 ± 19.85 ng/ml in cases with severe ascites, 60.09 ± 18.05 ng/ml in moderate ascites cases, 36.1583 ± 15..06 ng/ml in mild ascites cases and 19.79 ± 12.29 ng/ml in cirrhosis cases without ascites. The difference in mean serum prolactin level was significant among different severity of ascites. The serum prolactin level was 81.08 ± 18.04 ng/ml in cases with advanced hepatic encephalopathy, 56.89 ± 15.05 ng/ml in cases with mild hepatic encephalopathy and 26.16 ± 13.99 ng/ml in cirrhosis cases without encephalopathy. Conclusion: Serum Prolactin levels showed positive correlation with Modified Child Pugh Score and Fibroscan in predicting the severity of disease.