The aim of the study detects the relation between serum ferritin and hepcidin with hepatitis C virus One hundred subjects (men and women) were taken in the current study. Forty-four subjects without infection used like control group and fifty-six subjects with hepatitis C virus. The results demonstrate high significant (P < 0.05) elevate in levels of serum ferritin and hepcidin in subjects with hepatitis C compare with health subjects. It was concluded that there is positive relationship between serum ferritin, hepcidin and hepatitis C.