Background: The present study was undertaken for evaluating serum vitamin D levels and its relation to diabetic peripheral neuropathy in type 2 diabetic patients. Materials & methods: A total of 100 type 2 diabetic patients were enrolled in the present study. Complete demographic and clinical details of all the patients was obtained. Clinical examination was carried out in all the patients and data was recorded. assessment of peripheral neuropathy was done by neuropathy disability score (NDS) and nerve conduction studies. The NDS was established by bilateral examination of the pin–prick sensation, temperature sensation, vibration test, and Achilles tendon reflex. Serum levels were obtained and vitamin D levels were evaluated using autoanalyser. Correlation of vitamin D levels was done using auto-analyser. All the results were recorded and analysed using SPSS software.