The research dealt with the study of secondary amenorrhea and secondary amenorrhea and its relationship to the physiological and hormonal status of women, which is one of the gynecological diseases with serious effects on women with this disease, because cardiovascular diseases, osteoporosis, diabetes, infertility and mental state disorder are among the most important complications of this disease. . The study was conducted on (40) serum samples from Secondary Amenorrhea patients after their condition was diagnosed through clinical and laboratory tests. The ages of the patients ranged between (12-42) years. The levels of the hormones LH, FSH, E2, and Prog were measured, and the results were the presence of high significant differences (P <0.05) when measuring the level of hormones LH, FSH, E2, and Prog in the blood serum of the patients with secondary menopause and compared them with their concentrations in the control group. There were high significant differences (P <0.05) in serum levels of LH, FSH, and E2 hormones. The presence of high levels of the hormone LH, FSH, in the serum of most patients. The presence of a decrease in the levels of the hormones E2 and Prog in the serum of the majority of patients.