Inroduction: Gestational diabetes mellitus has emerged as a global public health problem with an exceptionally high estimated prevalence.The addition of biomarkers to predictive models of GDM may improve the ability to identify women at risk of GDM prior to its development.SETTING: SCB Medical College, Cuttack, Odisha, India. Objective: To study the role of NF-kB and DNA-methylation as biomarkers in gestational diabetes mellitus. Material And Method: Eligible participants were >18 years old with singleton pregnancy, plan to carry the pregnancy to term, and planned to deliver at our institution.Data was collected using in-person interviews and antenatal checkups. Information was collected on sociodemographic characteristics The study was a prospective observational study and enrolled 100 pregnant women after screening for the exclusion criterias. DNA extraction followed by Restriction fragment Length Polymorphism (RFLP) and DNA-methylation quantification was done in Molecular research unit (MRU). Results And Conclusion: We found the prevalence of GDM to be 34%.Three types of polymorphism were found. The most common type was ins/ins type (53%) followed by del/del type (32%) and ins/del type in only 15 %..This study provides the evidence that NF-kB polymorphism and global DNA-methylation has no predictive value for screening GDM.