Viral infection like Hepatitis B is a major global health issue and also for death it is a leading cause. Therefore, our current research was performed to understand the seroprevalence of HBV infection.methods:332 health care employees 168 were nursing personnel, 66 were laboratory technicians and 98 were paramedical staff were included. All the required information’s are obtained in the form of questionnaire. All the HCW’s were divided into 3 groups based on completion of vaccine schedule. Group A are those who completed full dose of vaccine and within 5 – 10 yrs group B were those who had partially completed vaccine and more than 10 yrs being vaccinated. Group c is those who never knew about vaccination and not vaccinated. Blood sample and serum separated for ELISA for Hepatitis B.Title >10 IU/ml of anti-HBs was deemed defensive. Results: Out of 332 serum samples, 152 (45.2%) were females, 182 (54.81%) were males. Among 332 HCW’s 298 (89.75%) were vaccinated, with 223 (67.16%) completely vaccinated and 75 (22.59%) were partially vaccinated and 34 (10.24%) were unaware of vaccination. among the 332 HCW’s 6 (1.80%) were found to be positive. Among the 223 vaccinated Hcw’s, 202 (90.58%) were fully vaccinated (group A) have anti HBs titre> 10 IU/ml, protective antibody level is higher in vaccinated group than partially vaccinated group. Conclusion: This study highlights the need for the HCW to be educated, vaccinated and maintains a stringent hospital policy in order to maintain the consistency of the health care system.