Background: To evaluate the role of vitamin D deficiency in benign paroxysmal positional vertigo. Materials and Methods: One hundred and twenty eight patients of benign paroxysmal positional vertigo of both genders were included. Serum 25-hydroxyvitamin D (25-OHD) was measured using ELISA method and concentration less than 20 ng/ml was considered as deficiency. Patients were divided into 3 groups. Group I (treatment group -with serum 25- OHD deficiency) received Epley maneuver + supplemental vitamin D and group II (nontreatment group - with serum 25-OHD deficiency) received only Epley maneuver therapy. Group III (non-treatment group - with normal vitamin D level) also received only Epley maneuver therapy. Results: Group I comprised of 18 males and 26 females, group II 20 males and 22 females and group III 21 males and 21 females. The mean(±SD) vitamin D level at baseline, 2 months and 6 months in group I was 11.4 ± 1.6 mg/ml, 34.1 ± 4.16 mg/ml and 35.9 ± 1.8 mg/ml respectively. In group II was 10.9 ± 1.4 mg/ml, 10.5 ± 2.01mg/ml and 11.6 ± 3.2 mg/ml. In group III was 33.5 ± 1.6 mg/ml, 34.8 ± 3.2 mg/ml and 35.2 ± 4.1 mg/ml respectively. The difference was statistically significant (P< 0.05). It was found that the intensity of BPPV decreased in group I, increased in group II and decreased in group III over the period of 2 months and 6 months. The difference was found statistically significant (P< 0.05). Conclusion: The correction of vitamin D deficiency in BPPV provides additional benefit to rehabilitation therapy regarding duration of improvement. Supplementation of vitamin D should be considered in patients with frequent attacks of BPPV, especially when serum vitamin D is subnormal.