Aim of work To assess the cut-off endometrial thickness and role of colour Doppler in differentiation between benign and malignant uterine condition in postmenopausal bleeding patients. Results: In our study, 6 mm is the cut-off point of endometrial thickness for differentiating malignant from benign cases with sensitivity of 65.62% and specificity of 100%. There was a statistically significant difference in endometrial thickness measured by TVUS between benign and malignant lesions with a tendency towards a thicker endometrium in the malignant condition. Conclusion: TVS with color doppler can ascertain the etiology of postmenopausal bleeding. With good specificity and high negative predictive value TVS can be employed as a first line screening test in females with postmenopausal bleeding.