Background: To evaluate the levels of serum amylase, lipase and creatinine phosphokinase in prognosis of organophosphorus poisoning. Materials & methods: A total of 50 subjects were enrolled. Complete demographic and clinical details of all the subjects were obtained. Blood samples were taken and serum amylase, lipase and creatinine phosphokinase levels were evaluated. Correlation of these biochemical variables were assessed with the outcome of organophosphorus substance consumed. Results were subjected to statistical analysis by using the SPSS 23.0 version for statistical analysis. A P value of ˂0.05 was considered to be statistically significant. Results: Mean age of the patients was 25.8 years (27 males and 23 females). Most of the patients were either farmers or housewives. Mean serum amylase among subjects who survived and expired was 96.3 IU/L and 284.8 IU/L respectively. Mean serum lipase among subjects who survived and expired was 94.8 IU/L and 211.7 IU/L respectively. Mean serum creatinine phosphokinase among subjects who survived and expired was 584.7 IU/L and 1574.8 IU/L respectively. Conclusion: Serum Amylase, Lipase and Creatine Phosphokinase Levels were significant.