Background: Salivary gland tumors are rare head-and-neck neoplasms. They demonstrate dual cell differentiation and morphologic overlap. Dual cell differentiation requires histomorphological study and immunohistochemistry to diagnose. p63 is a selective immunohistochemical marker expressed in nuclei of myoepithelial cells and basal duct cells in normal salivary glands and aid in the diagnosis of of salivary gland tumors by highlightining the biphasic nature of the tumors. Extent of p63 positivity can be a useful predictor of clinical outcome and could help in the aggressive mode of treatment in certain types of salivary glands tumors.To study the role of p63 in the diagnosis of salivary gland tumors. Material and Methods: The present study was conducted at Malla Reddy Institute of Medical Sciences in the Department of Pathology on the surgically resected salivary gland specimens received for routine histopathological evaluation. A total of 60 cases of salivary gland tumors were included in the study. It is a retrospective study carried out from January 2018 to December 2021, on surgically resected salivary gland tumor specimens and Immunohistochemical analysis with p63. Results: A Total of 60 Salivary gland tumors were studied with different cytomorphology and mixed architectural patterns. In the present study, most frequent age groups were between 40 and 60 years.. All tumors except Warthin's were femalepredominant. The parotid gland was the most common site, with adenoid cystic carcinoma mostly seen in smaller salivary glands. Pleomorphic adenoma and Mucoepidermoid carcinoma were frequent tumors seen. Standard techniques of IHC with p63 antibody were carried out on all the 60 Salivary gland tumors. All the benign tumors were positive for p63. Malignant tumors with basal cell involvement, such as Adenoid cystic carcinoma 6/7 cases showed p63 positivity with varying intensities. Clear, intermediate and squamous cells of all Mucoepidermoid carcinoma were p63 positive. p63 was negative in Acinic cell carcinoma. Differential localization of p63 in various neoplasms was observed which has given information on myoepithelial cells. Conclusion: P63 is an important Immunohistochemical marker which aids in diagnosis of salivary gland tumors and also helps in localization of myoepithelial cells by differential expression in various salivary gland tumors