Regeneration in endodontics is an evolving field that uses tissue engineering concepts to regenerate pulp, cementum, enamel, and dentinal structures. Regeneration was initiated by Nygaard-Ostby with the introduction of blood-clot-associated pulpal healing and apical closure. For an ideal regeneration Dental stem cells, scaffolds, growth factors, sterile environment and controlled release of growth factors are essential. Irrigants, medicaments, and antimicrobial agents have been used for canal disinfection but for stem cells to survive and proliferate, growth factors must be released at the proper concentration in both irrigants and medications. Nitric oxide is regarded as a potential therapeutic agent for its role in wound healing, antimicrobial effect, angiogenesis, and tissue remodeling. Hence, the use of NO as an adjuvant in the regeneration process is being explored. Several modalities, like NO-releasing nanomatrix gel, NO donors, or nitric oxide-releasing compounds (NOCs), are used to deliver nitric oxide within the root canal. This review paper discusses how nitric oxide aids in pulpal tissue regeneration.