Introduction:Colorectal cancer (CRC) is the third most diagnosed malignancy and the fourth leading cause of cancer-related deaths in the world. P53 protein is a nuclear biomarker that is most investigated for its predictive value in CRCs.Aim: to evaluate the relationship between P53 expression and colorectal carcinoma. Materials and methods:In this study we investigated the expression of P53 in colorectal carcinoma using immunohistochemistry on 60 cases collected retrospectively from Department of Pathology, Faculty of Medicine, Zagazig University.Results:30/60 (50%) of the cases showed positive P53 expression, the commonest Dukes stage was stageA which forms 25/60 (41.7%), There was a high statistically significant difference (p<0.001) between the studied subgroups as regard histopathology, age, lymph node metastasis, distant metastasis and DUKES, a significant difference (p=0.002) between the studied subgroups as regard lymph vascular invasion. Conclusion: P53 overexpression in colorectal carcinoma was associated with DUKES stage, lymph node metastasis, distant metastasis, and lympho-vascular invasion.