Background: The aim of the present study is to the comparison of viral load suppression and increase in CD4 count in the HIV reactive patients by the new regimen (TLE) within shorter period compared to the old regimen (TLD). Material and Methods: The study includes newly diagnosed100 HIV patients. The data collected included age, sex, regimen used by the HIV patients, CD4 count and Viral Load count. Out of these 100 patients 50 of them were initiated on old ART regimen (TLE) and other 50 of them were initiated on new ART regimen (TLD). The CD4 count was estimated using Flowcytometry (BD FACS CountTM) and Viral Load count was estimated by RT-PCR technique (Abbott HIV-1 Assay). Results: After 6 months out of 50 patients who were on TLE (old regimen),40 of them showed CD4 count,>560 cells/mm3 and Viral Load target not detected and 10 of them showed CD4 count >460 cells/mm3 and Viral Load 300 copies/ml. and out of 50 patients on TLD (new regimen), 45 of them showed CD4 count, >650 cells/mm3 and Viral Load target not detected and 5 of them showed CD4 count >500 cells/mm3 and Viral Load 150 copies/ml. After 12th month out of 50 patients who were on TLE (old regimen), showed CD4 Count > 680 cells/mm3 and 45 of patients showed viral load target not detected and 10 showed 210 copies/ml. After out of 50 patients who were on TLD (new regimen) showed CD4 Count > 800 cells/mm3 and Viral load target not detected. Conclusion: Present study concluded that, the new regimen (TLD) is well- tolerated and more efficient in viral suppression than old regimen (TLE).The immunological monitoring is essential for the overall success of treating, monitoring and control of HIV.