Abstract: Purpose: Varicocele may be associated with normozoospermic or oligozoospermia. Much dispute still exists regarding the diagnosis, management, and pathophysiology of spermatogenesis alterations associated with varicocele. We analyzed the two adipokines and tumor necrosis factor (TNF-α) in the seminal plasma of men with normozoospermic and oligozoospermia with or without varicocele. Materials and Methods: Where the study performed in the Biology department, College of Science, University of Babylon, and an academic clinic in 70 consecutive patients with varicocele and 35 controls without varicocele. for the period of September 2019 to February 2020. The age of the patients was 21-50 years. The samples were divided according to WHO (2010) into two groups normozoospermia and oligizoospermia. There were 35 patients of men with primary infertility were diagnosed with varicocele and 35 without varicocele divided into four groups as oligozoospermia with varicocele (VO) (no. 13), oligozoospermia without varicocele (CO) (no. 21), and fertile controls as normozoospermic with varicocele (VN) (no.22) and normozoospermic without varicocele (CN) (no. 14) Results: Increased seminal resistin and TNF-α (P< 0.05) levels were observed in the of men with varicocele and in those with normozoospermic and oligozoospermia. In this study reported adipokines levels in human seminal plasma in normal weight, overweight, and obese patients. seminal leptin and resistin level in overweight, and obese patients group was significantly higher (P< 0.05) than that in normal weight group, but there was significant difference in the seminal TNF-α levels between these two groups.