ABSTRACT Aim: Purpose of our research is to study the release of nickel as well as chromium ions from orthodontic wires in patients who are using teeth whitening mouthwashes.Methodology: In vitro, experimental study was carried out on 50 orthodontic wires of same length of SS, and NiTi. The samples were immersed in Oral B and Listerine (20 each) for 1, 6, 24, and 168 h. 10 samples immersed in distilled water served as the control group. Atomic absorption spectroscopy served to compute the number of released ions.Results: Nickel ions were released from both wires at all time-points; the highest amount was in Listerine and the lowest in Oral B mouthwashes. The process of release of chromium from the SS wire was the same as that of nickel. However, the release trend in NiTi wires was not uniform.Conclusion: Listerine caused the highest release of ions. Oral B showed the lowest amount of ion release.