Background: ACL injuries are commonly associated with sports injuries and road traffic accidents. Incidence of ACL ruptures is estimated to range from 38-78 % per 100000 person years. The primary aim of ACL reconstruction is to restore the function of the ACL and native kinematics of the knee. ACL reconstruction restores the stability of the knee joint and protects the menisci and joint surfaces from further damage. The most preferred graft tissues for ACL reconstruction are hamstrings in view of better cosmesis, satisfactory results and to minimize postoperative mobility. However, use of peroneus tendon graft is increasing in view of simplicity of the technique. Peroneus longus graft harvest is possible due to synergistic action of peroneus longus and peroneus brevis. Even some studies suggest that peroneus brevis is more effective evertor than peroneus longus, justifying harvest of peroneus longus tendon. Materials & Methods: We have done 26 cases of ACL injuries treated with arthroscopic ACL reconstruction using peroneus longus autograft at GSL medical college between Jan 2020 to Dec 2020. Cases were followed up for a period of 1 year and have been evaluated based on Lysholm, AOFAS and FADI score systems. Results: In our study, all 26 patients have completed follow up for 1 year (19 male, 7 female). At final follow up anterior drawer test showed normal findings in 80percent, while 20 percent of all examined patients had 1+ anterior laxity. Conclusion: Peroneus longus tendon is a promising autograft for ACL reconstruction with advantage of simplicity of harvesting technique, larger graft diameter, minimal graft complications.