ABSTRACT: Gingival crevicular fluid (GCF) is an inflammatory exudate derived from the periodontal tissues. It is composed of serum and locally generated materials such as tissue breakdown products, inflammatory mediators, and antibodies directed against dental plaque bacteria. Gingival crevicular fluid (GCF) contains a rich array of cellular and biochemical factors which have been shownto indicate the metabolic status of the periodontium1 .Since GCF is composed of serum and locally generated components such as tissue breakdown products, inflammatory mediators and antibodies in response to oral microorganisms present in the dental biofilm thus it offers great potential to reflect the response that the cells and periodontal tissues promote to attempt regaining homeostasis and also how certain periodontopathogens co-opt these response mechanisms to promote bacterial survival within the gingival crevice and pocket1 .