One of the oldest medicinal systems, the Siddha system has many mono-herbal, poly-herbal, herbo-mineral, and animal-based formulations for a variety of diseases.Agnimugachooranam (AMC) is one of the medicines described in Agathiyarvaithiyarathinasurukam 360, for the treatment of Rheumatoid Arthritis (Uthiravaathasuronitham).Thin layer chromatography studies are one of the most important identity tests in most pharmacopoeial monographs. High-performance thin layer chromatography (HPTLC), a TLC extension, is a simple, fast, and effective technique for quantitative compound analysis.Heavy metal toxicity has far-reaching ill effects on living organisms.Aflatoxin is naturally occurring mycotoxins produced by fungi. The existence of aflatoxin in herbal medicines is a well-known issue. The detection of aflatoxin with good sensitivity and also that is reliable in complex.