Background: Breast cancer is always known to be a major health problem globally. Though many advancements are achieved in treatment strategy for breast cancer, still the patient survival rate is not promising for all the affected patients. Hence there is an emerging need for an alternative medicine for treating cancer patients. Objective: Thus, we aimed to understand the possibility of using commonly available red seaweed, Gracilaria corticota in treating breast cancer. To analyse this, we aimed to check the seaweed extract’s efficiency in breast cancer cell lines, MCF 7 using proteome wide analysis. Method: The MCF7 cells were treated with red seaweed extract and incubated for 48 hrs. Further the treated cells were scraped and lysed to extract the proteins. The extracted proteins were estimated and further subjected to proteomic profiling. To analyse the differential protein expression and identification of the proteins, two-dimensional electrophoresis coupled with mass spectrometry was adapted. Results: We found upon seaweed extract treatment in MCF7 breast cancer cell line, important apoptotic proteins were deregulated. These proteins include the down regulation of BCL2,BCL2L1 and MCL1 and upregulation of BAX protein upon treatment when compared to untreated cells. Conclusion:This is a preliminary study to suggest that seaweed extract have very good potential against the breast cancer cell line by initiating the apoptotic proteins and target the cancercells. As well proteome-based approaches help us to identify the differentially expressed proteins and provide us the basic understanding about the tumorigenesis process which will eventually help us for better treatment strategy.